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Antibody Platform

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Mouse Monoclonal Antibody Production

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  • 服务简介

    Antibodies are an important tool in the diagnostic and therapeutic field. Monoclonal Antibodies are monotype of antibody which can bind to a specific epitope on target antigen. The first step to produce monoclonal antibodies is to synthesize an immunogen, and  then inject the immunogen into a selected host animal. With the development of antibody preparation techniques, hybridoma technology are the preferred method due to its maintaining inherent antibody structural and functional information.

    In recent years, rabbit monoclonal antibody production using phage display and mouse monoclonal antibody production using hybridoma technology have been as suitable methods to produce antibodies. What are the advantages of using mouse derived antibodies? Mouse has been the main host for antibody production since the utilization of hybridoma technology. This method has many advantages as following: the hybridoma cell line will not adapt well to in vitro conditions; they might be essential for experiments in which MAb are used in vivo in mice; downstream purification may decrease antibody activity; do not influence antigen-binding capacity and important biologic functions. In addition, such an approach allows optimal in vivo affinity maturation of the humoral response. High-affinity antibodies can be immortalized by hybridoma derivatization, resulting in permanent cloning. At the same time, the combination of humanized immunoglobulin (Ig) gene is of great significance for the study of human function analysis.

    Biotyscience offers mouse monoclonal antibody production service, we can provide a one-step service from antigen synthesis to antibody purification, covering human testing , animal testing and other molecular testing. Our products all provide quality inspection reports, for your later experiment escort. 


    服务流程
    服务内容

    1


    Antigen preparation

    Antigen designn

    Antigen synthesis and expression 

    2


    Immunization and ELISA titration


    Conventional or accelerated immunization protocol

    ELISA Titer testing

    3


    Fusion screening and subcloning


    Fusion clone screening by indirect ELISA

    Addition screening assays available upon request

    Subclone screening


    4


    Production and purificatio

    Small-scale antibody purification

    Isotype identification

    Antibody purification

    Antibody validation by indirect ELISA

    5


    Deliverables

    2-5 ELISA positive clones

    Purified antibodies, 1-5 mg/clone

    Remaining Antigens

    Project Report




    服务优势

    Consistent: Batch-to-batch consistency

    Sensitive and specific: High Sensitivity and high specificity to samples

    Cutting-Edge Technology & Facility

    Well-established generation platforms

    Various Antibody Production Technologies

    A wide range of antigens

    Antibody validation approaches

    Expert-guided custom project design


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