Insect baculovirus expression vector system (BEVS) belongs to the eukaryotic expression system, and it is an expression system with high safety. Insect baculoviruses are capable of transferring genetic information into mammalian cells, and, consequently, baculovirus-based vectors can be used as expression vectors for mammalian cells. The baculovirus is capable of infecting nondividing cells, lacks cytotoxicity, and is not infectious in humans. This is especially convenient because innate immunity to the majority of human viruses limits the use of the vectors based on these viruses. Baculoviruses provide a means to overcome this difficulty; i.e., recombinant viruses of a nonhuman origin can be constructed to serve as vectors for gene transfer. Humans lack both antibody and cell-mediated innate immune memory against baculoviruses because baculoviruses are only infectious for insects.
Biotyscience baculovirus/Insect cell expression system was developed by our in-house team of scientists tol enable virus production and expression of recombinant proteins from baculovirus-infected insect cells. This expressionl system supports improved protein solubility and incorporation of post-translational modifications. Our high throughput insect expression & purification platform is also available for smallscale production to fit your target screening projects.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1. Gene Synthesis And Codon Optimization
1.1 Customer provides protein /DNA sequence or plasmid
1.2 Codon optimization and gene synthesis according to customer requirements
2. Vector Construction
2.1 Cloning cDNA into expression vectors
2.2 Plasmid sequencing
2.3 Plasmid preparation
3. Virus Packaging And Amplification
3.1 Virus packaging
3.2 Virus amplification
4. Protein Expression And Purification
4.1 Cell infection
4.2 Pilot expression and purification trial
4.3 Purity detected by SDS-PAGE
5. Large-scale Expression And Purification
5.1 Purity detected by SDS-PAGE.
5.2 Using Western blotting to detect the molecular weight of protein
Large capacity: ability to carry large gene fragment; advantage in large protein expression.
High safety: baculovirus has strict species specificity.
High expression efficiency: the protein can be efficiently expressed in the late-stage infected cells.
Professional Technical Team: We can provide customers with professional technical support and solutions.
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com