Expression of proteins in yeast is a common alternative to prokaryotic and higher eukaryotic expression. Yeast cells offer many of the advantages of producing proteins in microbes (growth speed, easy genetic manipulation, low cost media) while offering some of thel attributes of higher eukaryotic systems (post translational modifications, secretory expression). Several yeast protein expression systems exist in organisms from the genera Saccharomyces, Pichia, Kluyveromyces, Hansenula and Yarrowia.
At the heart of any yeast expression system is the expression vector. Vectors that integrate into the host chromosome are most widely used because of their mitotic stability in the absence of a selection. However, episomal expression vectors exist for some yeast systems. Expression vectors typically contain a strong yeast promoter/terminator and a yeast selectable marker cassette. Most yeast vectors can be propagated and amplified in E. coli to facilitate cloning and as such, also contain an E. coli replication origin and ampicillin selectable marker.
Biotyscience provides comprehensive and high-quality customized recombinant protein production services with a yeast expression system. Our mature protein expression systems and advanced recombinant protein production technology support us, so we can offer industry-scale protein services to meet high-throughput and largescale production requirements.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Gene synthesis, Cloning, Plasmid Prep
1.1 Gene synthesis and Codon optimization
1.2 Subcloning into an appropriate expression vector
1.3 Plasmid Prep and DNA QC
2.Transformation of yeast expression strains
2.1 Transform plasmids into appropriate yeast expression strains
2.2 Amplification and culture of the above 5-10 positive clones
2.3 SDS-PAGE
3.Screening of yeast expression strains
3.1 Detection of recombinant protein producation
4.Protein expression evaluation and optimization
4.1 Scale up protein expression using optimized conditions
5.Small-scale protein expression and purification
5.1 The recombinant proteins were purified by affinity chromatography, ion exchange chromatography, and gel filtration.
5.2 SDS-PAGE
High efficiency and short cycle
Strict quality control
Customization: quantity, purity, tags, and endotoxin levels are customizable.
One-stop services, including protein purification, protein sequencing, protein labeling and related services.
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com