Biotin-labeled peptides have many important applications in immunology and histochemistry, such as affinity purification1 and FRET-based flow cytometry2, solid-phase immunoassays3, and receptor localization4, that exploit the high affinity of streptavidin and avidin for biotin.Biotinylated Peptides are designed for screening ELISA (Enzyme-linked immunosorbent assay) assays that require immobilized peptides onto streptavidin coated 96-well plates, membranes, or glass slides.
Biotyscience is committed to providing the biotechnology industry with the most complete biotinylation development services. We have extensive expertise in biotinylation, various antibodies, peptides and splicing technologies. We can provide one-stop service to maximize support for the development and manufacture of biotinylated peptides. Meanwhile, we have a quality assurance system and experimental validation process to ensure the quality and reliability of the biotinylated peptides we provide.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
Biotinylated peptide synthesis is usually carried out using solid-phase synthesis techniques, and the following are the general steps of the method:
1. Protective Group Selection
Before starting the peptide chain synthesis, an appropriate protective group needs to be selected to protect the amino acid residues that are not to be reacted.
2. Biotin Labeling Group Introduction
Biotin molecules are introduced at specific positions in the synthesized peptide chain.
3. Peptide Chain Synthesis
Using solid-phase synthesis techniques, the peptide chain length is gradually expanded by sequential addition of protective group removal, coupling of amino acid monomers, and washing steps according to the designed peptide sequence.
4. Deprotection And Purification
After synthesizing the complete peptide chain, it is necessary to remove the protecting groups and purify the target peptide.
5. Validation of Biotinylated Peptides
Validation can be done by mass spectrometry, SDS-PAGE gel electrophoresis and other techniques to confirm the presence and purity of the labeled peptide fragments.
Strict quality control: MS and HPLC identification results are provided for each peptide product
Superior technical support: assist client from design to delivery
Optimized Chemistries: Expertise in hundreds of cross linking modifications, activation and/or conjugation with reactive cross linkers with optimized labeling ratio
A rapid and high flux platform for peptide synthesis
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com