VHH antibodies, first discovered in 1993, are derived from camelids (such as alpacas, llamas, and camels) heavy chain only antibodies,and are the smallest antigen-binding fragments. Nanobodies, also known as single-domain antibodies (sdAb/VHH), are heavy-chain only antibodies that are naturally devoid of light chains, found in camelids such as llamas and in cartilaginous fish, including sharks.
Leveraging its state-of-the-art phage display technology, Bioltyscience has become a leading entity in VHH antibody discovery and is proud to provide high-quality antibody discovery services to renowned institutions and companies worldwide. At Bioltyscience, your VHH antibody discovery program is handled by a trusted person.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1. Antigen preparation /Antigen validation
Antigen can be prepared by BioIntron or provided by our client.
2. Alpaca Immunization and PBMC Isolation /preservation
2.1 Pre-immune bleed.
2.2 The ELISA titer after 2nd, 3rd and 4th immunization will be monitored and the report will send to client.
2.3 The PMBC after 2nd, 3rd and 4th immunization will be extracted and stored in Trizol.
3. VHH Phage Library Generation
3.1 RNA extraction and cDNA preparation
3.2 VHH amplification
3.3 Electro competent E. coli
3.4 Library Diversity and capacity measurement.
3.5 Clone NO counting and sequencing to measure the capacity of the library.
4. Library Biopanning and Screening
4.1 Liquid or solid phase panning.
4.2 ELISA verification, identification and evaluation of positive clones screening.
4.3 Positive clone sequencing and sequencing result analysis.
5. VHH-Fc Antibody Production (optional)
5.1 Gene synthesis, subcloning, plasmid preparation
5.2 Transient expression and purification
5.3 QC analysis
Nanoantibody libraries with higher diversity and greater coverage
Rich experience in antibody screening to ensure the best antibody screening to the greatest extent
Perfect antibody evaluation system, including ELISA, WB, FACS, etc
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com