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Antibody Platform

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Biotyscience Inc

E-mail biotyscience@gmail.com 

            info@biotyscience.com

Tel 400-669-8850


Alpaca Nanobody Production

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  • Service

    VHH antibodies, first discovered in 1993, are derived from camelids (such as alpacas, llamas, and camels) heavy chain only antibodies,and are the smallest antigen-binding fragments. Nanobodies, also known as single-domain antibodies (sdAb/VHH), are heavy-chain only antibodies that are naturally devoid of light chains, found in camelids such as llamas and in cartilaginous fish, including sharks. 

    Leveraging its state-of-the-art phage display technology, Bioltyscience has become a leading entity in VHH antibody discovery and is proud to provide high-quality antibody discovery services to renowned institutions and companies worldwide. At Bioltyscience, your VHH antibody discovery program is handled by a trusted person.


    For other services please contact our customer service department.

    Website: www.biotyscience.com  Phone: + 400-669-8850  Email: biotyscience@gmail.com


    Details

    1. Antigen preparation /Antigen validation

    Antigen can be prepared by BioIntron or provided by our client.

    2. Alpaca Immunization and PBMC Isolation /preservation

    2.1 Pre-immune bleed.

    2.2 The ELISA titer after 2nd, 3rd and 4th immunization will be monitored and the report will send to client.

    2.3 The PMBC after 2nd, 3rd and 4th immunization will be extracted and stored in Trizol.

    3. VHH Phage Library Generation

    3.1 RNA extraction and cDNA preparation

    3.2 VHH amplification

    3.3 Electro competent E. coli

    3.4 Library Diversity and capacity measurement.

    3.5 Clone NO counting and sequencing to measure the capacity of the library.

    4. Library Biopanning and Screening

    4.1 Liquid or solid phase panning.

    4.2 ELISA verification, identification and evaluation of positive clones screening.

    4.3 Positive clone sequencing and sequencing result analysis.

    5. VHH-Fc Antibody Production (optional)

    5.1 Gene synthesis, subcloning, plasmid preparation

    5.2 Transient expression and purification

    5.3 QC analysis


    Highlights

    Nanoantibody libraries with higher diversity and greater coverage

    Rich experience in antibody screening to ensure the best antibody screening to the greatest extent

    Perfect antibody evaluation system, including ELISA, WB, FACS, etc


    Workflows

    Alpaca Nanobody Production.png

    Ordering Information

    Website: en.biotyscience.com

    Phone: +86-400-669-8850 

    Email: biotyscience@gmail.com


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