Stable cell lines play a pivotal role in biological research, widely employed in applications such as the production of recombinant proteins and monoclonal antibodies, drug screening, and gene function studies. Compared to traditional antibody production methods, utilizing a stable cell line overcomes many production issues and inconsistencies that come with hybridoma production or standard transient expression.
With years of expertise in protein expression research, Biotyscience possesses extensive experience in the development of stable cell lines. We are proficient in enhancing the production capacity of stable cell lines, thereby improving experimental outcomes. We provides a comprehensive, one-stop service from gene sequencing to the delivery of stable overexpression cell lines and reporter gene cell lines, emphasizing our commitment to delivering high-quality solutions for our clients.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Gene Synthesis
Simply provide us with the host cell information, RefSeq NM_* accession number, or the gene sequence.
2.Vector Construction And Plasmid Preparation
Subcloning into appropriate vectors.
3.Lentivirus Packaging And Production
High-efficiency lentivirus systems enable the stable integration of target genes into the host genome.
4.Lentivirus Transduction And Clone Screening
Screening for optimal single cell cloning with limited dilution.
5.Clone cryopreservation and QC
5.1 Stable cell validation by FACS or WB
5.2 Cell banking
5.3 Mycoplasma testing
Extensive experience with both GPCRs and Ion Channels
Choose from transiently transfected cells or stable cell lines
Access to MaxCyte STX electroporation system for robust multi-plasmid transfection for bulk transiently-transfected frozen cells
Ability to qualify cells for assay performance on a large range of platforms
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com