Bacterial protein expression are widely used expression system with low production costs, leads to high scalability, makes these systems an attractive option for the production of recombinant protein. E. coli are commonly used to store and replicate plasmids of all types, so E. coli expression system continues to dominate the bacterial expression systems and remain to be the preferred system for laboratory investigations and initial development in commercial activities.
Bacterial protein expression requires the insertion of the open reading frame into an expression vector, routinely a plasmid vector and the transferral of this vector into bacterial cells. The cells are then cultured and induced to express the desired protein. The cells are harvested by centrifugation, samples prepared and proteins detected by PAGE and subsequent staining of the gel with Coomassie Brilliant Blue or silver stain or by immunoblotting.
Many factors can impact protein expression and purification, Biotyscience’s protein expression experts offer custom bacterial expression services with one-step solutions, from gene synthesis to protein purification and characterization. Biotyscience's custom bacterial expression platform is optimized for low costs, high scalability of recombinant protein. We offer the service from the sequence to protein purification in 3-4 weeks. What’s more, we offer protein refolding technology for recovering biologically functional proteins from inclusion bodies. We will do our best to express high-quality protein to meet your experiments.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
Phase | Specification | Timeline |
Gene Synthesis | Sequence optimization Gene synthesis | 1 week |
Vector Construction | Cloning into expression vector | 1 week |
Transformation and Expression | Transformation Low-temp expression Protein expression evaluation Protein expression and optimization | 1-2 weeks |
Ni-NTA purification | Optimize purification conditions Large scale expression and purification | 1-2 weeks |
QC | SDS-PAGE and WB | 1 week |
Optimized enzyme for expression
Verification of the authenticity of the subcloned gene
Free for Protein analysis and project proposal
Ni-NTA or glutathione beads purification
High efficiency expression bacterial strain
Mini-induction to over-express the target protein
Test by SDS-PAGE and WB
Protein Sequence → Gene Synthesis → Vector Construction → Protein Expression → Protein Purification → Quality Control
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com