Rabbit polyclonal antibody is a type of polyclonal antibody produced by immunizing rabbits. Polyclonal antibodies are groups of antibodies produced by multiple B cell clones stimulated by immunogen in immunized animals. Rabbit is one of the most commonly used animal models because its immune system has a strong immune response to the antigen, and it can quickly produce high-affinity polyclonal antibodies after inoculation with the antigen. Obtaining polyclonal antibody (pAbs) is a well-knownand the most usedprocedureis rabbit models[1].
Our company has a professional antibody development and production team, which can provide customers with one-stop antibody preparation services including antigen design-peptide synthesis, protein expression-animal immunity-antibody purification - antibody labeling - immunological detection and other projects. According to the specific experimental needs of customers, we can provide polyclonal antibody preparation services for mice, rabbits, rats, chickens, sheep and other animal species, and the prepared polyclonal antibodies can be used for ELISA, Western Blot, IHC, IF, IP and other applications.
Biotyscience also provides value-added services such as antibody labeling, antibody pair screening, kit development, and immunological testing.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Antigen Preparation
Work with customers to select appropriate antigens or proteins to ensure research needs are met.
2.Purification
2.1Protein A purification
2.2Antigen affinity purification (As per customer's reques)
3.Rabbits Immunization
Immunization of selected antigens into rabbits. Multiple immunizations are required to enhance the immune response of the antibody.
3.1Choose the animal: New Zealand White rabbit.
3.2Primary immunization: the antigen is mixed with an adjuvant, which can enhance the immune response.
3.3Multiple immunization: multiple booster immunization over a period of several weeks or months.
3.4Serum collection: Blood is collected from the rabbit's ear vein and serum containing antibodies is isolated.
4.Antibody Purification
Isolation and purification of antibodies from collected serum.
5.Antibody Performance Evaluation
The purified antibody performance evaluation, including specificity, affinity and sensitivity and other indicators. The specificity and affinity of the antibody were verified by ELISA and Western blot.
According to customer requirements, antigens can be supplied by the customer or designed and produced by us
Independent optimization and development of immune adjuvants: stable, efficient, short cycle
Efficient price, high antibody production, to meet customer demand for high-quality antibodies
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com