Polyclonal antibodies (pAbs) remain a widely utilized tool in research and diagnostics. They identify specific antigens, principally protein antigens, and are used in Western blots, radioimmune assays (RIAs), enzyme-linked immunoabsorbent assays (ELISAs), indirect and direct fluorescent antibody test, hemaglutination tests, immunohistochemistry (IHC), immunoprecipitation (IP) assays, immunodiffusion, affinity chromatography, enzymology, and isolation of gene products[1].
Our company has a professional antibody development and production team, which can provide customers with one-stop antibody preparation services including antigen design-peptide synthesis, protein expression-animal immunity-antibody purification - antibody labeling - immunological detection and other projects. According to the specific experimental needs of customers, we can provide polyclonal antibody preparation services for mice, rabbits, rats, chickens, sheep and other animal species, and the prepared polyclonal antibodies can be used for ELISA, Western Blot, IHC, IF, IP and other applications.
Biotyscience also provides value-added services such as antibody labeling, antibody pair screening, kit development, and immunological testing.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Preparation of Immunogen
1.1The recombinant protein was obtained by molecular cloning vector and induced expression in Escherichia coli, and could be used as immunogen directly after purification and identification.
1.2Small molecular weight proteins or compounds, such as small molecular weight, need to be conjugated vectors to modify the molecule to become an immunogenic antigen.
2.Immune Animal
The adjuvant is mixed with the antigen and injected into the goat. Three immunizations are required, with the goat producing enough antibodies after each immunization.
3.Blood Collection
After the last immunization, a blood sample of the goat is collected. Antibodies were obtained after serum isolation.
4.Antibody Labeling
Antibodies are combined with other markers such as fluorescent dyes and enzymes for subsequent detection and analysis.
5.Antibody Identification
The specificity and affinity of the antibody are detected by Western blot, ELISA and other methods to ensure the quality and availability of the antibody.
According to customer requirements, antigens can be supplied by the customer or designed and produced by us
Independent optimization and development of immune adjuvants: stable, efficient, short cycle
Efficient price, high antibody production, to meet customer demand for high-quality antibodies
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com