Chimeric antibodies are molecules made up of domains from different species[1]. Chimeric antibodies have been used to identify the domain or residues that are involved in effector functions such as antibody dependent cellular cytotoxicity or complement dependent cytotoxicity[2].
The antibody prepared by us has good integrity and long retention time, which not only retains the high specificity and affinity of parent mouse antibody, but also reduces the murine component by about 70%. The human Fc segment can effectively mediate biological effects, such as antibody-dependent cell mediated cytotoxicity, complement dependent cytotoxicity, etc. Moreover, it can freely select the type, subtype, class, subclass, size, structure domain and glycosylation site of antibodies to take advantage of their different biological and physical and chemical characteristics. Chimeric antibody expression composed of highly efficient eukaryotic expression vector and constant region of human antibody can allow the insertion of different variable regions of mouse monoclonal antibody, which is relatively simple and shorten the operation and development cycle.
Biotyscience has many years of experience in antibody preparation and provides mammalian cell chimeric antibody expression services, including homologous full-length antibody and heterologous chimeric antibody expression. It covers the comprehensive gene sequence of human, mouse, rabbit, dog, sheep, monkey, etc., and delivers microgram chimeric antibodies.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
Human/Mouse Chimeric Antibody
Chimeric antibodies can be generated by fairly straightforward genetic engineering, by joining the immunoglobulin (Ig) variable regions of a selected mouse hybridoma to human Ig constant regions, and be used as such or as a first stage towards further humanization.
Human/mouse Chimeric Antibody Preparation Process
1.The spleen containing B cells from an immunized mouse is collected. The B cells are fused with myeloma cells to construct hybridoma and isolate a clone producing antigen-specific IgG.
2.The DNA sequences coding mouse VH and VL are then isolated from the clone, as well as the DNA sequences coding human immunoglobulin constant regions from human cells.
3.Mouse/human chimeric genes are constructed by genetic engineering and transfected into mammalian cells.
4.The clone revealing a high level expression of chimeric IgG is selected and the IgGs are purified from the culture supernatant.
Guarantee high success rate and fast delivery speed;
Service: reasonable price, according to the comprehensive analysis of customer needs, to provide customers with satisfactory results;
Professional technical support, ensure strong, thoughtful and timely pre-sales and after-sales service support, at any time for you to answer any questions;
Complete upstream and downstream services: Provide one-stop technical services from antigen preparation, antibody expression/chimeric antibody expression, antibody purification, endotoxin control, large-scale preparation, etc., saving customers valuable time and funds;
Biotyscience Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com