Fab fragment antibodies (Fab = fragment antigen-binding region) are a valuable tool in molecular biology research and diagnostics. These antibodies are generated through the process of papain digestion, which cleaves the antibody molecule and produces Fab fragments.To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments. Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescent proteins or alkaline phosphatase. These bifunctional proteins having both antigen-binding capacity and marker activity can be obtained from transformed bacteria and used for one-step immunodetection of biological agents[1].

Biotyscience is committed to the research and development of genes, recombinant proteins, antibodies, phage libraries and other biologics, and is committed to providing customers with one-stop CRO technical services.

For other services please contact our customer service department.

Website: www.biotyscience.com  Phone: + 400-669-8850  Email: biotyscience@gmail.com

1.Gene synthesis And Codon Optimization 

Starting from antibody sequence

2.Vector Construction

2.1 Cloning into expression vectors

2.2 Plasmid sequencing

2.3 Plasmid production

3.Expression and Purification

3.1 Transient transfection of HEK293/CHO cells

3.2 Antibody purification

3.3 Polishing if needed

4.QC analysis

4.1 SDS-PAGE

4.2 UV

4.3 SEC-HPLC

4.4 Endotoxin tests

Guarantee high success rate and fast delivery speed;

Service: reasonable price, according to the comprehensive analysis of customer needs, to provide customers with satisfactory results;

Professional technical support, ensure strong, thoughtful and timely pre-sales and after-sales service support, at any time for you to answer any questions;

Complete upstream and downstream services: Provide one-stop technical services from antibody preparation, antibody sequencing, antibody labeling and modification, saving customers valuable time and funds.

High freeze-thaw stability, in vivo half-life of 2-3 weeks, effectively control the risk of immunogenicity;

Unique and flexible molecular properties enable fine regulation of dual target action for better efficacy

Ordering Information

Website: en.biotyscience.com

Phone: +86-400-669-8850 

Email: biotyscience@gmail.com