Mammalian cell multi-protein expression refers to a technology that simultaneously expresses two or more proteins in mammalian cells, aiming to meet the needs for structural analysis of multi-protein complexes, functional research, and recombinant protein production.
Biotyscience offers professional Mammalian Cell Protein Co-Expression Service, tailored to address the complexities of co-expressing multiple proteins and assembling functional multi-subunit complexes—challenges that are critical for advanced research and biopharmaceutical development.
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Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
Phase | Steps | Time-consuming |
Expression Vector Construction | 1. Vector selection and modification 2. Multi-gene insertion | 2 weeks |
Cell Transfection and Screening | 1. Host cell selection 2. Cell transfection 3. Stable cell line construction | 2 weeks |
High-Expression Cell Screening | 1. Fluorescent screening 2. Monoclonal purification 3. Expression verification | 2-3 weeks |
Large-Scale Culture and Protein Purification | 1. Selection and optimization of the culture system 2. Protein purification and identification | 2-3 weeks |
Protein Function Integrity
Multi-Protein Co-Expression
Efficient Screening System
Strong Scalability
Suitability for Complex Protein Expression
Reference
Assur, Z., Hendrickson, W. A., & Mancia, F. (2012). Tools for coproducing multiple proteins in mammalian cells. Methods in molecular biology (Clifton, N.J.), 801, 173–187.
Qin, C., Xiang, Y., Liu, J., Zhang, R., et al. (2023). Precise programming of multigene expression stoichiometry in mammalian cells by a modular and programmable transcriptional system. Nat. Commun. 14, 1500.
Wenjun Guo, Mengmeng Wang, Lei Chen. (2022). A co-expression vector for baculovirus-mediated protein expression in mammalian cells. Biochemical and Biophysical Research Communications, Volume 594, 69-73.
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com