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Proteinase K (High Conc.)

Cat NoBIOP2717
Conjugate
Type其他蛋白
SourceP.Pichia
TagTag Free
Size50 mg
ApplicationImmunogen
FormatLyophilized
ConcentrationPlease refer to the vial lable for the specific concentration.
BufferSupplied in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, 5mM CaCl2, pH 7.2-7.4.
SpeciesTritirachium album
StorageStore at –20 degree. Avoid repeated freeze/thaw cycles.
SynonymsProteinase K
Purification> 95% by SDS-PAGE.
MolecularWeight
Description
BackgroundProteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0 . Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction . Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA .
We can offer labeled proteins/antibodies using a broad range of intensely fluorescent dyes and labels including FITC, Biotin, Alexa Fluor, Rhodamine B, Cy, Co-Au.