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Cat No | BIOP2717 |
Conjugate | |
Type | 其他蛋白 |
Source | P.Pichia |
Tag | Tag Free |
Size | 50 mg |
Application | Immunogen |
Format | Lyophilized |
Concentration | Please refer to the vial lable for the specific concentration. |
Buffer | Supplied in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, 5mM CaCl2, pH 7.2-7.4. |
Species | Tritirachium album |
Storage | Store at –20 degree. Avoid repeated freeze/thaw cycles. |
Synonyms | Proteinase K |
Purification | > 95% by SDS-PAGE. |
MolecularWeight | |
Description | |
Background | Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 60°C and a pH between 7.5 and 12.0 . Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction . Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA . |