Small molecules, or haptens do not have immunogenic but can induce antibody responses when coupled to carrier proteins. The hapten then reacts specifically with the antibodies generated against it to produce an immune or allergic response. Thus, although the hapten cannot elicit an antibody response on its own, it can bind with carrier and act as an antigen[1].
After years of practice, we have rich experience in the design of small molecule antigen coupling and its antibody preparation. By using a variety of technical means, the small molecule hapten was artificially modified to improve its immunogenicity while retaining its characteristic epitopes, and the preparation and coupling process of small molecule antigen antibody were improved.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
Immunogen Design
Synthesis or purification of target small molecules as immunogen. It can be coupled with a protein carrier to enhance the immunogenic properties of the immunogen.
Small Molecule Coupling
Different coupling modes are adopted according to different groups. When preparing the complete antigen, the specificity of the small molecule structure must be maintained. If necessary, a certain length of carbon chain can be introduced between the small molecule and the carrier protein, which can facilitate the production of antibodies against small molecules.
Identification methods: animal immunoassay, electrophoresis, TLC, IR, UV scanning, nuclear magnetic resonance and mass spectrometry
Immune Animal
The usual method of animal immunization is to mix the complete antigen and adjuvant into emulsion first, and the female BALB/c mice with sc 6-8 weeks are immunized for the first time. Booster immunization is performed once every 14 days, and serum titer is monitored. If the titer meets the requirements, booster immunization can be performed.
Cell screening, Fusion and Subcloning
The common method for establishing hybridoma cell lines is cell fusion on day 3 after shock immunity. The fusion cells must be screened, and the positive cells obtained were cloned and cultured to obtain hybridoma cell lines that were both genetically stable and able to secrete single antibodies.
Clonal Amplification
Monoclonal cells are collected from positive monoclonal hybridoma cells and amplified to ensure their single origin and increase antibody production
Purification and Assay
Small molecule antibodies were purified by centrifugation, osmosis chromatography and affinity chromatography. This process removes other contaminants and improves the purity of the antibody.
High success rate: Rich experience in small molecule coupling and immunity to ensure a high success rate of the experiment;
High efficiency: high coupling efficiency, complete the experiment in a short cycle, saving customers precious time;
High stability: strict quality control system, standardized management system, to ensure reliable results;
Satisfactory service: tailor-made experimental program for customers to meet the needs of customers' experimental results.
Complete upstream and downstream services: Provide one-stop technical services from antibody preparation, antibody sequencing, antibody labeling and modification, saving customers valuable time and funds.
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com