Antibody humanization & antibody characterization can reduce the immunogenicity of monoclonal antibodies derived from xenogeneic sources and improve their activation of the human immune system by replacing non-human antibody frameworks with human ones. It is a very important step in the therapeutic antibody discovery process.
Antibody humanization is an efficient approach to eliminate or reduce the immunogenicity of these antibodies[1]. Depending on the principle of action, humanized antibodies can be divided into four categories: chimeric antibody, reshaped antibody, resurfacing antibody and fully humanized antibody.
With a complete protein expression purification platform, stable cell line construction platform and phage display technology platform, we can provide you with a complete solution for antibody development. For humanized monoclonal antibody development services, our company can provide 9~18 humanized sequences selection, CDB transplantation, and professional quality evaluation system, in vitro biological activity analysis of humanized antibodies and chimeric antibodies, FACS and SPR affinity detection, and homologous species cross-reaction analysis.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Construct the parental structure model
2.Chimeric antibody-antigen affinity validation
3. Design of humanized antibodies
(1)3D structural modeling of antibodies
(2)Back mutation
(3)Vector construction and plasmid preparation
4.Evaluation of humanized antibodies
5.Expression and purification of humanized antibodies
6. Screening of humanized antibodies
Project experience: A number of antibody humanization and affinity maturation projects have been completed.
Multi-species antibody humanized antibody development: dog, monkey, mouse, camel, alpaca, chicken, etc.
Combined with bioinformatics and cytology, the antibody design was aided by computer technology.
The degree of humanization is high, and the affinity of the antibody can reach nM and pM levels after maturation.
Provide a variety of humanization technologies: including chimeric antibodies, CDR transplantation, SDR transplantation, chain replacement, etc.
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com