Recombinant proteins are proteins produced from transfecting artificially-made DNA into a host cell. The mammalian cell expression system has the function of post-translational modification and protein folding, which makes the recombinant proteins expressed by it more close to the natural higher biological protein molecules in terms of molecular structure, physical and chemical properties and biological functions, and more likely to acquire the same biological activity as the natural molecules.
Mammalian cell expression exhibits the highest level of protein processing, which is important for complete biological activity.l Therefore, the mammalian expression system is suitable for expressing complicated, modified, functional proteins/antibodies; secreted or membrane proteins, such as recombinant glycoproteins (vaccines and enzymes), hormones, antibodies, interleukins and lymphokines.
Biotyscience protein expression technology team has rich experience in CHO and HEK293 cell expression, and can provide a variety of special requirements such as label removal, endotoxin removal, serum-free suspension culture, etc., to provide services for customers.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Gene Synthesis And Codon Optimization
1.1 Customer provides protein /DNA sequence or plasmid
1.2 Codon optimization and gene synthesis according to customer requirements
2.Vector Construction
2.1 Cloning cDNA into expression vectors
2.2 Plasmid sequencing
2.3 Plasmid preparation
3.Protein Expression And Purification
3.1 Transient transfection of HEK293/CHO cells
3.2 Protein purification
3.3 QC analysis
4.Large-scale Expression And Purification
4.1 Purity detected by SDS-PAGE.
4.2 Using Western blotting to detect the molecular weight of protein
Extensive experience in mammalian protein expression
A variety of fusion tag vectors and hosts to ensure high soluble mammalian protein expression and activity.
One-stop services, including mammalian cell culture, mammalian protein expression, protein purification, protein sequencing, protein labeling and related services.
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com