Mammalian protein production platforms have had a profound impact in many areas of basic and applied research, and an increasing number of blockbuster drugs are recombinant mammalian proteins.More than 50% of therapeutic proteins approved and on the market are produced using mammalian cells, mainly due to the ability of mammalian cells to synthesize proteins that are similar to those naturally occurring in humans with respect to molecular structures and biochemical properties.
The mammalian cell expression system has the function of post-translational modification and protein folding, which makes the recombinant proteins expressed by it more close to the natural higher biological protein molecules in terms of molecular structure, physical and chemical properties and biological functions, and more likely to acquire the same biological activity as the natural molecules. Biotyscience protein expression technology team has rich experience in CHO and HEK293 cell expression, and can provide a variety of special requirements such as label removal, endotoxin removal, serum-free suspension culture, etc., to provide services for customers.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1. Gene Synthesis And Codon Optimization
Simply provide us with the protein/gene sequence or plasmid.
2. Vector Construction
2.1 Cloning cDNA into expression vectors
2.2 Plasmid sequencing
2.3 Plasmid preparation
3. Protein Expression And Purification
3.1 Transient transfection of HEK293/CHO cells
3.2 Protein purification
3.3 QC analysis
4. Large-Scale Expression And Purification
Higher activity: Mammalian systems that achieve complex glycosylation modifications and higher protein activity
Instantaneous transfection Instantaneous transfection of cells, rapid and small amount preparation of proteins/antibodies
Independent research and development: Independently developed high expression vector, self-built cell house, experimental success rate >95%
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com