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Conjugation Kit

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生物素3 末端DNA标记试剂盒

Cat NoBIOK2010
Conjugate
TypeKit
SourceN/A
Size20次
ApplicationNB; SB; EMSA; colony hybridization
FormatLiquid
ConcentrationPlease refer to the vial lable for the specific concentration.
BufferSupplied in PBS.
StorageStore at -20 degree. Avoid repeated freeze/thaw cycles.
SynonymsBiotin 3 End DNA Labeling Kit;生物素3 末端DNA标记kit
Purification
NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
MolecularWeight
DescriptionTerminal Deoxynucleotidyl Transferase(TdT)可以在不依赖于模板的情况下催化在DNA的3 -OH端加上dNTP的反应。关于TdT催化双链DNA末端加dNTP的反应效率,3 端突出的双链DNA要比平端或3 端缩进的双链DNA高很多。TdT催化单链DNA末端加dNTP的反应效率要比双链DNA高很多。在适当的条件下,TdT也可以催化RNA 3 末端加NTP的反应。
Background生物素3 末端DNA标记试剂盒(Biotin 3 End DNA Labeling Kit)是一种通过Terminal Deoxynucleotidyl Transferase(TdT)把生物素标记的dUTP添加到DNA 3 末端的试剂盒。通常DNA的3 末端被标记后不会干扰杂交反应,也不会干扰基于序列特异性蛋白结合的EMSA检测;因此生物素标记的DNA探针可以用于常规的Northern、Southern、EMSA(即gel shift)以及colony hybridization或原位杂交等。