Magnetic beads and protein/antibody labeling technology is also known as immunomagnetic bead labeling technology. It uses highly uniform magnetic microspheres as carriers, coated with ligands on the surface, and uses specific reactions to separate target substances from mixed substances. Magnetic beads exhibit superparamagnetic properties in the presence of an external magnetic field, where they can rapidly aggregate and settle.
Biotyscience provides customized magnetic bead labeled antibody and protein services, as well as customized antibody labeling technology services, you can choose the required service items.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
Experimental Procedure
1. Magnetic bead pretreatment (activation): washing, magnetic separation, resuspending magnetic beads. (Commonly used amino magnetic beads and carboxyl magnetic beads.)
2. Antibody binding (coupling): dilute the antibody with binding buffer to prepare the antibody working solution, and combine it with the magnetic bead suspension for magnetic separation. (The labeling coupling reagent is EDC/NHS.)
3. Antibody cross-linking reaction: suitable for single elution target antigen experiment.
4. Antigen precipitation: antigen adsorption, washing, transfer.
5. Antigen elution: denaturing elution method and non-denaturing elution method.(The method can be selected according to the detection requirements.)
6. Magnetic bead post-treatment.
Technical Advantages
Simple operation, no need for large instruments and equipment
Fast separation speed
Stable results, high repeatability
No effect on the properties and functions of the separated components
Professional development team with rich project experience in the preparation of colloidal gold
Efficient service process that updates progress in real time from submission of requirements to delivery of final results.
Strict quality control to ensure product stability.
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com