Monoclonal Antibodies are monotype of antibody which can bind to a specific epitope on target antigen. The first step to produce monoclonal antibodies is to synthesize an immunogen, and then inject the immunogen into a selected host animal. Once the immunogen causes an immune response, the b-cells in the spleen are extracted and fused with the myeloma cells. Healthy splenocytes do not survive indefinitely in cell culture, so use cell fusion to create a hybridoma cell line. Biotyscience offers monoclonal antibody preparation services, including murine monoclonal antibody preparation and rabbit monoclonal antibody preparation. We provide different animals according to the customer requirements and conduct experiments.
Mouse Monoclonal Antibody Production
Five 6-8-week female Balb / c mice
(1) Suitable for WB / IHC / IF / IP;
(2) Guaranteed antibody preparation service;
(3) ELISA antibody pairs preparation service;
(4) IVD antibody customization.
Rabbit Monoclonal Antibody Production
Compared with mice monoclonal antibodies, rabbit monoclonal antibodies have high affinity and more abundant recognized epitopes, which can recognize the outstanding advantage of having no immunogenic antigen in mice. During the past few decades, different techniques have been developed to produce monoclonal antibodies. Single B cell cloning technology offers many advantages compared to other methods and has been used to generate monoclonal antibodies from different species including rabbits. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity.
(1) 2 rabbits immunization
(2) Antibody heavy- and light-chain sequences
(3) Matched antibody pairs for ELISA
(4) High affinity and specificity
Small Molecule Antibody Production
Small molecules with low molecular weight are not usually immunogenic, but can induce antibody responses when coupled to carrier proteins. Suitable macromolecules are usually albumins, thyroglobulins, haemocyanins and polylysine. The hapten then reacts specifically with the antibodies generated against it to produce an immune or allergic response. The production of specific antibodies against haptens is important both for assays of hormones and drugs or for therapeutic applications in tumour therapy. Therefore, Approaches in choice of both carrier protein and conjugation methods are very important. Biotyscience is professional in preparation of antibodies of food safety residues and trace residues in biologics. Small molecule antigen and antibody production services are provided to meet your specific needs.
(1) Drugs, Allergens, Growth Promoters and etc
(2) Tag Antibodies
(3) Matched antibody pairs for ELISA and LF
(4) High affinity and specificity
Antibody Humanization Production
Humanization is a method for generating antibody therapeutics compatible with the human immune system. And humanized antibodies are an effective way to eliminate or reduce the immunogenicity of these antibodies and become a powerful tool in the treatment of human diseases. So far, various methods have been innovated for humanization of non-human antibodies and to improve their affinity, specificity and other properties. Common methods for producing humanized antibodies include framework-homology-based humanization, germline humanization, complementary determining regions (CDR)-homology-based humanization and specificity determining residues (SDR) grafting, as well as advantages and disadvantages of each of these methods and their applications. Our service delivers a panel of humanized variants from your clone, guaranteeing to produce a variant with comparable activity to the parent antibody.
(1) Determination of expression yields
(2) Activity testing
(3) Matched antibody pairs for ELISA and LF