Antibodies are essential for structural determinations and functional studies of proteins, but antibody production is limited by the source and purified antigen. Polyclonal antibodies are derived from multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against a single molecular species of antigen recognize multiple epitopes on a target molecule resulting in signal amplification in indirect immunoassays, including immuno-electron microscopy.
An antibody’s specificity results from the antigen-binding site formed within the variable regions—regions of the antibody that have unique patterns of amino acids that can only bind to target antigens with a molecular sequence that provides complementary charges and noncovalent bonds. Biotyscience can offer different animal sources of polyclonal antibody preparation services to meet your specific experimental needs.
Polyclonal antibodies are a crucial tool in the fields of research and diagnostics, with a wide range of applications in immunology, biochemistry, and molecular biology. Polyclonal antibodies are produced by immunizing animals with a specific antigen, resulting in the production of a heterogeneous mixture of antibodies that can recognize different epitopes on the antigen. The production of polyclonal antibodies typically involves immunizing animals such as rabbit with an antigen. And we can guarantee polyclonal antibodies are suitable for applications such as Western blotting, ELISA.
(1) High titer and affinity
(2) Tag Antibodies
(3) Matched antibody pairs for ELISA and LF
The production of polyclonal antibodies typically involves immunizing animals such as rabbits, mice, rats, guinea pigs, goats, or sheep with an antigen. The animal’s immune system responds by producing antibodies, which can be collected from the animal’s serum. Polyclonal antibodies have many advantages, such as their high specificity and versatility. The production of polyclonal antibodies involves several key steps, including immunization, blood collection, serum preparation, and antibody purification.
(1) High titer and affinity
(2) Low cost
(3) Suitable for applications: WB, IHC, IF.
Our comprehensive polyclonal antibody services include production of custom polyclonal antibodies in rat and offers one-stop solutions for your custom polyclonal antibody production, from antigen design, peptide synthesis, and animal immunization, to antibody purification and QC with ELISA test. One or two rats are immunised using our standard protocol. For each rat pre-immune serum is collected prior to immunisations, and two test bleeds are made over the course of the immunisation schedule.
(1) High affinity
(2) Higher Purified pAb
(3) High ELISA Titers of 1:100,000
(4) Suitable for applications: WB, IHC, IF.
Antibodies or immunoglobulin (IgGs) are heterodimer proteins produced by the B lymphocytes of the immune system of vertebrates. The association between the antibody and antigen involves a myriad of noncovalent interactions between the epitope and the paratope. The traditional way of producing antibodies is by immunizing the host animal using whole proteins that are purified from a biological source or produced via a recombinant technology. We can routinely accommodate polyclonal antibody projects in goat on special request. Goats are similar to rabbits in producing a strong response against a wide range of antigens. We recommend using goat as a host species for antibody production when large amounts of antiserum are desired, such as bulk quantity for product inventory, unique large lot for kit assembly, rabbits not applicable.
(1) High affinity
(2) Large scale production capability
(3) High ELISA Titers of 1:100,000
(4) Suitable for applications: WB, IHC, IF.
Bigger animals such as horses,sheep, pigs and also rabbits and guinea pigs, wereused for the production of polyclonal antibodies, whilemice and rats were used as a source of spleen for theproduction of monoclonal antibodies. However，chickens, as a source of desired antibodies, represent an alternate animal system that offers some advantages with respect to animal care, high productivity and special suitability of avian antibodies for certain diagnostic purposes. Despite being an excellent counterpart to mammal IgG chicken IgY antibodies still represent an underused resource. Our custom antibody services includes a customizable option for chicken immunization, egg-collection, and titer analysis for chicken antibody production.
(1) High affinity
(2) Higher Specificity
(3) Lower background
(4) High yield