The clinical and commercial success of antibodies has led to the need for very large-scale production in mammalian cell culture. This has been particularly successful in the upstream part of the process where productivity of cell cultures has improved 100 fold in the last 15 years. In addition to improving process/cost efficiencies, a second key area has been reducing the time taken to develop processes and produce the first material required for clinical testing and proof-of-principle.
Biotyscience provides high affinity and specific antibody development services. Depending on your needs for different antibody quantities, we will save you development costs. At the same time, we also provides antigen preparation services, including DNA synthesis, plasmid construction, protein expression, protein purification, protein modification, peptide synthesis, peptide modification, peptide and carrier protein cross-linking, small molecule structure analysis and carrier protein cross-linking. If required, we also provide antibody purification and antibody identification services.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Gene Synthesis And Codon Optimization (Optional)
Starting from antibody sequence
2. Vector Construction
Cloning into proprietary, high-efficiency expression vectors
Plasmid sequencing
Large-scale plasmid preparation
3. Expression And Purification
Transient transfection of HEK293/CHO cells
Antibody purification
4. QC Analysis
SDS-PAGE,ELISA, and more (optional)
Obtain mg to gram amounts of purified antibody.
Production in culture eliminates mouse ascites serum immunoglobulins.
Production can begin immediately after subcloning, saving process time and a freeze cycle.
Serum-free conditions lowers contamination of bovine immunoglobulin.
We can formulate purified antibody in appropriate buffer or lyophilize so you can use immediately.
Rigorous characterization of the purified antibody helps to protect against lot-to-lot variability.
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com