Hybridoma technology has long been a remarkable and indispensable platform for generating high-quality monoclonal antibodies (mAbs). Hybridoma-derived mAbs have not only served as powerful tool reagents but also have emerged as the most rapidly expanding class of therapeutic biologics. With the establishment of mAb humanization and with the development of transgenic-humanized mice, hybridoma technology has opened new avenues for effectively generating humanized or fully human mAbs as therapeutics[1].
The company has a professional antibody research and development and production team, which can provide customers with one-stop antibody preparation services for antigen design - peptide synthesis, protein expression - animal immunity - antibody purification - antibody labeling - immune detection and other projects. According to the specific experimental needs of customers, we can provide monoclonal antibody preparation services for animal species such as mice and rabbits, and the prepared monoclonal antibodies can be used for ELISA, Western Blot, IHC, IF, IP and other applications.
Biotyscience also provides value-added services such as antibody labeling, antibody pair screening, kit development, and immunological testing.
For other services please contact our customer service department.
Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Antigen Design
1.1Define immunization strategy
1.2Perform peptide synthesis, gene synthesis, and protein production or opt for DNA immunization
2.Cell Fusion
2.1Collect splenocytes from 2 mice
2.2Perform cell fusion with a myeloma cell line to create hybridomas
3.Hybridoma Selection And Screening
3.1Select hybrid cells using HAT selection
3.2Expand and screen monoclones by ELISA or in the target application for the highest quality antibodies
4.Isolation And Selection Of The Best Monoclones
4.1Isolate monoclones through limiting dilution
4.2Expand and screen monoclones by ELISA or in the target application for the highest quality antibodies
The antibody has high purity, strong repeatability between batches, less impurity contamination and no animal virus contamination
Efficient price, high antibody production, to meet customer demand for high-quality antibodies
Hybridoma technology is very mature and the preparation process is complete
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com