Yeasts are attractive hosts for the production of heterologous proteins. Unlike prokaryotic systems, their eukaryotic subcellular organization enables them to carry out many of the post–translational folding, processing and modification events required to produce “authentic” and bioactive mammalian proteins. In addition, they retain the advantages of a unicellular microorganism, with respect to rapid growth and ease of genetic manipulation. The vast majority of yeast expression work has focused on the well–characterized baker's yeast Saccharomyces cerevisiae.
With the rapid development of genetic engineering technology, hundreds of foreign proteins have successfully expressed the advantages of the expression system, the composition of the system, and the way and expression characteristics of the expression system transformed by foreign genes. With the help of rich experience in protein expression, Biotyscience constantly improves the internal system to provide customers with efficient protein production.
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Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
1.Gene Synthesis Plus Codon Optimization
1.1Leading codon optimization system
1.2 Analysis and optimization of rare codons, codon bias and other factors
2.Vector Construction
2.1 Clone target gene into expression vector
2.2 Plasmid sequencing
2.3 Plasmid preparation
3.Transformation
3.1 Transforming Pichia pastoris, Saccharomyces cerevisiae
3.2 Various transformation methods
4.Protein Expression and Purification
4.1 Expression condition optimization
4.2 Protein expression evaluation
4.3 Various protein purification methods
5.Quality Control
5.1 SDS-PAGE
5.2 Western blot
5.3 Mass spectrometry
Mature system: It has a perfect yeast expression platform and mature protein expression system;
Service oriented: According to the comprehensive analysis of protein characteristics and customer needs, design different experimental schemes, and explore and optimize the overall expression system of customers.
Self-improvement: Always improve the operation steps of protein induced expression, and provide customers with high-quality protein products.
Ordering Information
Website: www.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com