Transient expression of proteins in mammalian cells is a key technique for many functional and structural studies of human and higher eukaryotic genes as well as for the production of recombinant protein therapeutics.
Transient expression by infiltrating Nicotiana benthamiana leaves with Agrobacterium tumefaciens carrying genes of interest is used in plant science and molecular pharming. Transgenic plants are generally used to obtain recombinant proteins or identify the localization of proteins. However, substantial time is required to generate transgenic plants, and the yield of the expressed protein is relatively low. Transient expression systems with virus-based vectors have the advantage of rapid and high-level expression of the recombinant proteins. The replication system of plant viruses results in high-level expression of foreign proteins within a few days. Thus, plant virus expression vectors are alluring. The plant systems used for transient expression include protoplasts, calluses, suspension cultures of plant cells, intact plants, as well as isolated plant organs or specialized plant tissues. With the development of protein expression techniques, a simple method has been developed, protein expression yield in human embryonic kidney (HEK)-293 cells can be enhanced with a brief treatment of DMSO solution.
By transiently expressing genes of interest, we can investigate their roles in biological processes without the need for stable transformation, and can rapidly assess of gene function in a high-throughput manner, enabling more efficient functional genomic studies. As a result, maximize the expression efficiency to achieve a higher expression yield.
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Phase | Specifications | Timeline |
Gene Synthesis | Gene synthesis Sequence optimization Subcloning into an expression vector | 3-4 weeks |
Transfection | Plasmid amplification and preparation Transfection Small scale protein expression | 2-3 weeks |
Transient protein expression | Large scale protein expression Affinity purification | 3-4 weeks |
QC | SDS-PAGE and WB | 1-2 weeks |
Optimized enzyme for expression
Verification of the authenticity of the subcloned gene
Free for Protein analysis and project proposal
Test by SDS-PAGE and WB
Wide range of mammalian expression systems
Large-scale production in a short timeline
Integrated solution from gene to protein
Protein Sequence → Gene Synthesis → Vector Construction → Protein Expression → Protein Purification → Quality Control
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Phone: +86-400-669-8850
Email: biotyscience@gmail.com