The baculovirus expression vector system (BEVS) platform has become an established manufacturing platform for the production of viral vaccines and gene therapy vectors
The baculovirus expression vector system (BEVS) platform has become an established manufacturing platform for the production of viral vaccines and gene therapy vectors, with many advantages, including manufacturing speed, flexible product design, inherent safety and scalability.
The BEVS platform is an efficient process for producing a wide variety of proteins in a streamlined manner. BEVS has successfully expressed thousands of proteins, however, not all proteins are suitable to express by this system. Many factors must be considered such as protein complexity, post‐translational modification, scale and cost collectively.
To start the BEVS process, a recombinant baculovirus is constructed comprising the desired gene. Then, the gene is cloned into a transfer plasmid, behind the strong polyhedrin or p10 promoter; however, these promoters are not very active in E. coli and, can be stable expression cassettes.
Insect cells are then co‐transfected with the transfer plasmid and parental AcMNPV DNA. The plasmid and parental DNA undergo homologous recombination to produce de novo recombinant baculoviruses. These baculoviruses are plated and individual plaques purified to isolate a single plaque of recombinant baculovirus, then, generate a high‐titer stock and establish a working virus bank (WVB) for protein production.
BEVS platform has many features, such as no live pathogens, single cell line for multiple, sequence specificity, flexible product design, inherent safety, narrow host range, low cost. Biotyscience offers a full range of custom baculovirus expression services.
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Website: www.biotyscience.com Phone: + 400-669-8850 Email: biotyscience@gmail.com
Phase | Specifications | Timeline |
Gene Synthesis and Construct Assembly | Gene synthesis Sequence optimization Subcloning into a baculovirus transfer vector | 1-3 weeks |
Baculovirus Generation | Co-transfection: the transfer vector and baculovirus DNA are co-transfected into insect cells. | 1 week |
Amplification | P0 stock infecte insect cells Amplify recombinant baculovirus titer | 1 week |
Expression Testing | SDS-gels, WB, or pull-downs | 1 week |
Protein Production | Larger volumes of insect cell culture | 1-2 weeks |
Optimized enzyme for expression
Free for Protein analysis and project proposal
Test by SDS-PAGE and WB
Fast -pandemic solution
Scalable to large capacities
Outstanding capability for complex protein
Expression of biologically active proteins
Protein Sequence → Gene Synthesis → Baculovirus Generation → Virus Amplification → Expression Testing → Protein Production
Ordering Information
Website: en.biotyscience.com
Phone: +86-400-669-8850
Email: biotyscience@gmail.com