中文English Call: 400-669-8850 | E-mail: biotyscience@gmail.com

Protein Platform

Location:Home >>Protein Platform

Contact us

Biotyscience Inc

E-mail biotyscience@gmail.com 

            info@biotyscience.com

Tel 400-669-8850


Stable Cell Line Development Services

  • Service
  • Details
  • Highlights
  • Workflows
  • Service

    Protein products are manufactured using cells derived from bacterial, mammalian, insect and yeast cells. The selection is largely dictated by the expression of the target gene, which determines the suitability of a particular cell type for protein expression. Stable cell lines are cellular models that harbor genetic modifications to express specific proteins or biomolecules of interest. 

    Stable cell line generation can improve product batch-to-batch consistency, and suit for more applications in research and biologics production. Stable cell line generation involves many steps, including choose the expression system based on efficiency to produce the desired gene or protein (such as CHO, Hybridoma or HEK 293 are typical platforms of choice), choosing a suitable vector comprising promoter and regulatory elements, transfection or transduction (introducing a vector into the host cell), selecting successfully transfected or transduced cell lines, and cloning and expansion of the selected cells.

    Biotyscience offers a full range of custom stable cell line development services to generate high yields of recombinant protein products. The vector is transfected in a monoclonal host cell line that will not only be able to deliver high titers and desired product characteristics, but also be transferable to manufacturing. 


    For other services please contact our customer service department.

    Website: www.biotyscience.com  Phone: + 400-669-8850  Email: biotyscience@gmail.com

    Details

    Phase

    Specifications

    Timeline

    Gene Synthesis and Construct vector

    Gene synthesis

    Sequence optimization

    Subcloning into a transfer vector

    1-3 weeks

    Transfection

    Transfect host cells with recombinant plasmids

    1-2 weeks

    Selection of Pool of Transfected Cells

    Select cells that are stable and producing protein

    2 weeks

    Screening and Selection

    Screen and select clones for high expression

    2 weeks

    Cell Line Characterization

    Validate each clone for stability

    1-2 weeks

    Expansion

    Expand clones

    1-2 weeks


    Highlights

    All projects are discussed in-depth before starting

    Free for Protein analysis and project proposal

    Test by SDS-PAGE and WB

    Scalable to large capacities

    Outstanding capability for complex protein

    Non-viral and viral-based delivery systems

    Cas9 technology for targeted gene editing

    High-throughput screening


    Workflows

    Protein Sequence → Gene Synthesis → Construct vector → Transfection → Selection of Pool of Transfected Cells → Screening and Selection → Cell Line Characterization → Expansion

    Ordering Information

    Website: en.biotyscience.com

    Phone: +86-400-669-8850 

    Email: biotyscience@gmail.com


    Can you introduce the product?

    How much do you charge?

    Are there any promotions now?

    请您留言

    Thank you for your attention, the current customer service staff is not online,

    提交